Precision Fiber Microtome Sectioning Method and Specimen Preparation

The Precision Fiber Microtome/fiber slicer is used to cut fibers or yarns into very small cross-sectional slices for microscopic observation of their tissue structure for further inspection or research.


Slicing method:

1. Turn the micrometer to the bottom;

2. Unscrew the two screws on the cover and remove the cover;

3. Pass the fiber through the small hole of the cover plate from below to expose a small section;

4. Tighten the screws on the cover plate, push the push plate to the side of the small hole, compress the fibers, and lock the nuts on the push plate;

5. Cut the fibers along the cover with a blade;

6. Turn the micrometer to reveal the required fiber thickness;

7. Drop collodion;

8. Use a blade to cut the fibers along the cover.

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Sample preparation:

(1) Fabrication of longitudinal fiber samples: Take a small bundle of samples, straighten them by hand, use the thumb and index finger of the right hand to pick up about 20-30 fibers, press the fibers at the clamping end on the glass slide, and cover them with the left hand. Place a coverslip and remove excess fibers to make the fibers attached to the slide straight, then place a drop of distilled water on each pair of top corners of the coverslip to stick the coverslip and increase the clarity of the field of view .

(2) Production of fiber cross-section sample slices: Generally, the Hastelloy slicing method is used, that is, a method of cutting fibers (or yarns) into thin slices with a precision Hastelloy slicer.


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