The study of the factors influencing and optimising the slicing accuracy in the Fiber microtome is an important research topic. The following are some of the factors that may affect the slicing accuracy of Fiber microtome.

1. Tool quality: The quality of the cutting tool has a direct impact on the cutting accuracy.

2. Tool wear: With increased use, the cutting tool wears severely, leading to inaccurate slicing.

3. Fibre moisture content: the moisture content has a direct effect on the hardness and elasticity of the fibres and can vary during the cutting process.

4. Type and morphology of the fibres: the morphology and structure of the fibres vary from material to material, which can lead to different degrees of cutting misalignment and inaccuracy.

5. Cutting speed: when cutting at high speeds, the forces and heat on the fibres also increase, affecting cutting accuracy.

Based on the above considerations, the cutting accuracy of the Fiber microtome can be optimised in four ways：

1. optimising the tool: choosing the right tool material and shape, carrying out proper maintenance and dressing to ensure the quality and performance of the tool.

2. Controlling the moisture content: controlling the moisture content of the fibres to prevent them from becoming too wet or dry helps to improve slicing accuracy.

3. Selecting appropriate parameters: Cut fibres optimally by adjusting cutting parameters, such as cutting speed and depth of cut, to achieve better cutting accuracy.

4. Using numerical simulation: By using numerical simulation techniques, the Fiber microtome is simulated and analysed to determine the optimal cutting parameters and cutting solutions to improve cutting accuracy.

In summary, by considering the above factors and using the corresponding optimisation strategies, we can effectively improve the cutting accuracy of the Fiber microtome and meet the requirements of different applications.