8 ways to get the most out of the fiber microtome
The fiber microtome is an instrument used to cut fiber-like material to produce thin, uniform sections for microscopic observation or other analysis. fiber microtome has many types and uses, but how can you get the most out of it? In this article, we will introduce 8 ways to help you improve the efficiency and quality of the use of the fiber microtome.
1. Choose the right type of fiber microtome. According to the nature and purpose of the substance you want to cut, choose the most suitable type of fiber microtome. For example, if you want to cut plant fibers, you can choose rotary or sliding type of fiber microtome; if you want to cut animal fibers, you can choose frozen or ultrasonic type of fiber microtome.
2. Adjust the parameters of the fiber microtome. Adjust the cutting speed, temperature, pressure and other parameters of the fiber microtome according to the thickness, hardness, humidity and other factors of the material you want to cut. This will ensure the quality and consistency of the slices and avoid damaging the substance or the instrument.
3. Prepare the substance for cutting. Before cutting, treat the substance appropriately to facilitate cutting and observation. For example, you can perform steps such as fixation, dehydration, embedding, and staining of the substance to enhance its stability and visualization.
4. Mount the cut substance. Mount the processed substance on the fiber microtome, taking care to fix it firmly to avoid moving or falling off. Also, make sure the distance between the substance and the blade is appropriate, not too close or too far.
5. Start cutting. According to the set parameters, start the fiber microtome and start cutting. Pay attention to the shape, thickness, integrity and other characteristics of the slice, and adjust the parameters or stop cutting in time to prevent problems.
6. Collect and save the slices. Collect the cut sections and place them in appropriate slides or containers, taking care to avoid contamination or damage. If not used immediately, store the sections in suitable conditions, such as cold, dry, and protected from light.
7. clean and maintain the fiber microtome. after each use, clean and maintain the fiber microtome promptly, remove residual substances and liquids, inspect the instrument for damage or wear, and replace or repair faulty parts. This will prolong the service life of the fiber microtome and ensure its performance.
8. learn and master the principle and operation of the fiber microtome. In order to better use the fiber microtome, you need to understand its working principle and operation, familiar with its various components and functions, master its common problems and solutions. This will improve your skills and confidence in using the fiber microtome.