What is the working principle of fiber microtome?

The fiber microtome is an experimental device used to prepare fiber slices. Its working principle mainly includes the following steps:


Preparation of fiber samples: First, the fiber samples to be cut need to be prepared. Typically, fiber samples need to be fixed, dehydrated, embedded, etc., so that they can maintain their morphological and structural integrity when sectioned.

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Slicing guide: place the treated fiber sample on the sample table of the microtome, adjust the cutting angle and position of the microtome so that the cutting direction is perpendicular or approximately perpendicular to the fiber direction.


Slicing process: Start the microtome, the slicing knife will move along the surface of the sample and rotate continuously during the cutting process, thus cutting the sample into thin slices. The rotation speed and cutting depth of the slicer knife can be adjusted as needed.


Collection of slices: After the slices are completed, the slices are collected and placed on a microscope slide for staining, washing, etc., so that the slices can be observed and analyzed under the microscope.


The cutting principle of fiber microtome is mainly based on rotary cutting technology. During the cutting process, the rotation speed and cutting depth of the slicing knife can be adjusted as needed to control the cutting speed and cutting depth, so as to obtain the desired slice thickness and shape. At the same time, in order to ensure the cutting quality, the blade of the slicing knife needs to be kept sharp and regularly maintained and replaced.


Fiber microtome has a wide range of applications in materials science, biomedicine and other fields, and can be used to prepare slices of various materials and biological samples, such as fibers, fabrics, paper, bone tissue, etc., providing important sample preparation for subsequent microscopic observation and analysis means.