Tips for using the small fiber microtome
The small fiber microtome is an instrument for cutting fiber samples. The following are some tips for use:
1. Sample preparation: Before cutting, the sample must be prepared. This includes fixing the sample on a slide in the correct position and treating the sample with appropriate antioxidants and/or fixatives to prevent sample distortion or disintegration.
2. Slice Thickness: Adjust the slice thickness to suit your experimental needs. Slice thickness can be accomplished by adjusting the controls on the slicer.
3. slice speed: slice speed may need to be adjusted depending on sample type and thickness. If the slicing speed is too fast, the sample may be damaged. If the slicing speed is too slow, uneven slicing may result. 4.
4. Slice angle: Make sure the slice angle is correct. For some experiments, slices parallel to the fibers are required, while for other experiments, slices perpendicular to the fibers are required.
5. Slice protection: When cutting and collecting samples, take care to avoid contamination of the sample or exposure to other environmental factors. After sectioning, slides can be used to move the sections to their proper position.
6. Maintenance: Periodic cleaning of the slicer and replacement of blades ensures optimal performance of the slicer. Cleaning and maintenance of the slicer should be performed according to the manufacturer's instructions.
These are some tips for using the small fiber microtome. Before using, read the manufacturer's instructions carefully and make adjustments as needed.